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Discussion

What is the reaction in DNA replication catalyzed by DNA ligase?

  • A.Addition of new nucleotides to the leading strand
  • B.Addition of new nucleotide to the lagging strand
  • C.Formation of a phosphodiester bond between the 3’-OH of one Okazaki fragment and the 5’-phosphate of the next on the lagging strand
  • D.Base pairing of the template and the newly formed DNA strand

Answer: C

DNA ligase catalyzes the formation of a phosphodiester bond between 3’-OH of one Okazaki fragment and 5’-phosphate of the next.

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