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Discussion

Which of the following is used to determine the appropriate stringency (Wallace rule)?

  • A.Tm = 2 x (No.of G:C base pair) + 4 x (No.of A:T base pair)
  • B.Tm = 4 x (No.of A:T base pair) + 2 x (No.of G:C base pair)
  • C.Tm = 4 x (No.of G:C base pair) + 2 x (No.of A:T base pair)
  • D.Tm = 69.3° + 0.41 (%)G+C -650/1

Answer: C

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